Strategies for biofilm remediation

Published: 7-Aug-2015

Biofilm creation presents a challenge to maintaining clean processes within GMP regulated industries as many factors are involved. A. Deal, D. Klein and P. Lopolito, Steris Corporation Life Science Division, describe a project aimed at seeing how diferent parameters affect biofilms

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In nature and in process systems, micro-organisms rarely exist as single cells or even as pure cultures, but rather as a monoculture or a mixed culture of different micro-organisms. Micro-organisms within a biofilm, such as Pseudomonas species, are commonly encased in a slimy matrix referred to as extracellular polymeric substances (EPS), which is essential for the micro-organisms’ survival.

The development and presence of the EPS is very important in increasing the micro-organisms’ resistance to environmental stresses, antimicrobial agents and cleaning agents. Therefore it is critical to remove the EPS prior to sanitisation or disinfection. Surface conditions, such as rouge, can also play a role in removal and disinfection so it is important to consider these when developing a remediation strategy.

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